Objective To establish a rapid and accurate molecular method for detecting Clostridium perfringens （C. perfringens） in food using fluorescent multi-enzyme isothermal rapid amplification （MIRA）.Methods Primers and probes based on the conserved sequence of the C. perfringens alpha toxin-coding gene plc were designed separately， and the fluorescence MIRA method was established and optimized. The genomic DNA of 17 non-C.perfringens strains were extracted for specificity verification， and the isolated DNA and bacterial solutions of different dilutions were used for sensitivity evaluation. Matrix interference testing with 4 types of food media was also performed to evaluate the stability of the method， and the actual sample detection results were compared with the standard method to verify its practicality.Results The fluorescent MIRA method for C. perfringens used in this study had high specificity. The results returned negative for other non-C. perfringens strains. MIRA also had high sensitivity， with detection limits of 1.05×10¹ fg/μL for genomic DNA and 7.5×10⁰ CFU/mL for pure C. perfringens cultures. It also had a strong anti-interference ability； the detection results were not affected by the matrix， and the detection time was short （approximately 20 min）.Conclusion This study developed a rapid， accurate， sensitive and specific fluorescent MIRA method for the molecular detection of C. perfringens in food.