重组酶恒温扩增技术在动植物源成分分析中的应用与研究进展
作者:
作者单位:

1.成都产品质量检验研究院有限公司,四川 成都 610000;2.空军通信士官学校,辽宁 大连 116600;3.四川省食品检验研究院,四川 成都 610000

作者简介:

史贇学 男 助理研究员 研究方向为食品安全与控制 E-mail:872551416@qq.com

通讯作者:

吴宇 男 高级工程师 研究方向为食品化工检测 E-mail:9934448@qq.com

中图分类号:

R155

基金项目:

国家市场监督管理总局科技计划项目(2021MK096)


Application and research progress of recombinase isothermal amplification technology in the analysis of animal and plant components
Author:
Affiliation:

1.Chengdu Institute of Product Quality Inspection Co., Ltd., Sichuan Chengdu 610000, China;2.Air Force Communication Non-commissioned Officer School, Liaoning Dalian 116600, China;3.Sichuan Institute of Food Inspection, Sichuan Chengdu, 610000

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    摘要:

    近年来,以肉制品和转基因农作物掺假掺杂为主的动植物源成分分析成为食品安全研究及监管领域关注的重点,对于靶向物检测水平的要求也逐渐提高。重组酶恒温扩增技术是一种具有实用性和发展前景的检测技术,包括重组酶聚合酶恒温扩增(RPA)和重组酶介导的恒温扩增(RAA)等,因其具备快速便捷、灵敏度高、特异性强等优点,广泛应用于动植物源成分检测及各类分析研究领域。本文综合近年来国内外相关研究,归纳了现有分析检测方法,从引物探针设计、外界影响因素、反应装置和检测途径4个方面对RPA/RAA技术的特点及应用进行概述,并对该领域发展趋势进行展望,为动植物源成分检测方法的完善和重组酶恒温扩增技术的应用提供参考。

    Abstract:

    Recently, the analysis of animal- and plant-derived components, mainly based on the adulteration and smuggling of meat products and genetically modified crops, has been a key focus in the field of food safety. Therefore, the requirement for analytical methods has been increasingly emphasized. Recombinase isothermal amplification is a novel and promising detection technique that currently includes recombinase polymerase amplification (RPA) and recombinase-aided amplification (RAA). This technique has been widely used in animal- and plant-derived component analysis and other detection and analysis fields owing to its speed, convenience, and high sensitivity and specificity. This paper summarizes relevant research conducted locally and abroad in recent years and concludes with an analysis of various detection methods. It also generalizes the characteristics and applications of RPA/RAA technology in four aspects: primer-probe design, external factors, reaction devices, and detection methods, and lays out the prospects for RPA/RAA development trends. Finally, this study also provides a reference for improving the analysis and detection methods of animal- and plant-derived components and promoting RPA/RAA technology.

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史贇学,王鑫,王峻,姚华,吴宇.重组酶恒温扩增技术在动植物源成分分析中的应用与研究进展[J].中国食品卫生杂志,2024,36(2):231-238.

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  • 收稿日期:2022-06-17
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  • 在线发布日期: 2024-05-24
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