Objective To understand the contamination levels of 24 per- and polyfluoroalkyl substances （PFASs） in fishes sold in Tianjin， and conduct characteristic analysis and health risk assessments.Methods The concentrations of PFASs were determined by ultra-performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS）. Comparison was performed using nonparametric tests. Health risk assessment was carried out by calculating the hazard quotient （HQ） values.Results A total of 103 marine fish and 70 freshwater fish samples were tested for PFASs contamination. Seventeen kinds of PFASs were detected in marine fish， with ∑PFASs concentrations ranging from 0.160 to 34.223 μg/kg. Fourteen kinds of PFASs were detected in freshwater fish， with ∑PFASs concentrations ranging from 0.158 to 8.690 μg/kg. PFASs detected in both types of fishes were predominantly long chain compounds， and the component with the highest detection rate in both types was all PFOS. The detection rate of F-53B（6/2） in freshwater fish was 14.286%. The concentrations of ∑PFASs and 8 individual PFASs differed significantly in the two fish types. The HQ values of PFOA， PFOS and PFNA were all below 1.Conclusion There is a certain degree of PFASs contamination in fishes sold in Tianjin， but the associated health risks are within acceptable limits， indicating an overall low safey concern.