Objective To assess the nutritional value of protein in the genetically modified rice with double antisense starch-branching enzyme（SBE）gene and its parental rice. Method The adopted nutritional value assessment methods, including chemical score (CS), amino acid score (AAS), protein digestibility-corrected amino acid score (PDCAAS), essential amino acid index (EAAI), nutritional index (NI), ratio coefficient of amino acid (RCAA) and score of RCAA (SRCAA) was applied to assess the protein nutritional value of the rice. Results The CS value of genetically modified rice and parental rice were 62 and 59, AAS value were 75 and 62, PDCAAS value were 65 and 56, EAAI were 79 and 80, NI were 11 and 8, SRCAA were 76.7 and 67.0, respectively. RCAA were the same. Conclusion The nutritional value of protein in the genetically modified rice with double antisense SBE gene were not greatly changed by the increase of resistant starch content in the rice. So, the nutritional value of protein in the genetically modified rice and its parental rice was substantial equivalent.