Objective To observe the effects of oats glucan on vascular endothelial active substances and inflammatory markers (CRP) in hypercholesterolemic rats. Methods Male SD rats were randomly divided into normal group (11 rats) administered with distilled water and high cholesterol group (33 rats) fed with high fat diet for 4 weeks. It was applied as an indicator for the success of inducing hypercholesterolemic model when the levels of fasting serum total cholesterol (TC) in the high cholesterol group was significantly higher than the control group. The hypercholesterolemic rats were regarded as the model group and then divided into three subgroups，high cholesterol control group and two oat glucan groups fed with different doses of oat glucan (500 and 1000 mg/（kg?d）). The rats in control groups were given water，and the rats in oar glucan groups were fed with oat glucan for another 6 weeks. Vascular endothelial function indexes, including nitric oxide (NO), endothelium (ET-1), thromboxane A2 (TXA₂), and inflammatory marker C-reactive protein (CRP) were tested. Results The levels of NO were increased and the levels of ET-1and TXA₂ were decreased in the oat glucan groups compared with the high cholesterol control group, and the levels of CRP was decreased with higher dose of oat glucan. Conclusion There might have some effects of oat glucan on improving the vascular endothelial function，inhibiting platelet aggregation，reducing inflammatory response and protecting vascular endothelial cells.