Objective To detect Pseudomonas aeruginosa in adult chicken,and provide basis for epidemiological investigation or for the control of related diseases.Methods Suspicious strains were isolated from 49 anus swab samples of adult chicken before slaughtering and examined by oxidase test,pyocyanin test and biochemical identification.Results Eight suspicious strains were isolated from anus swabs,and the positive rate was 16.3%.According to morphological observation,oxidase test,pyocyanin test and biochemical identification,8 suspicious strains were identified as pseudomonas aeruginosa.Conclusion Pseudomonas aeruginosa could be carried by healthy chicken.As a foodborn pathogen,pseudomonas aeruginosa do harms to the human.