Abstract Objective To characterize the etiological characteristics, molecular epidemiology and antimicrobial resistance of Vibrio parahaemolyticus from outbreaks cases in Shaoxing City from 2022 to 2023. Methods The Vibrio parahaemolyticus strains were isolated from five outbreak events from 2022 to 2023. Serotyping, molecular typing, drug resistance testing, virulence gene detection and bioinformatics analysis were performed by slide agglutination, pulsed field gel electrophoresis (PFGE), broth microdilution, multiple fluorescent PCR and whole genome sequencing methods. Results The 36 strains of Vibrio parahaemolyticus were isolated from 1 leftover dinner ingredient, 2 kitchen swabs (cloth and cutting board) and 33 clinical anal swabs associated with outbreak. These isolates comprised ten different serotypes, with O10:K4 being the predominant one. All strains were divided into 11 band types, and the main PFGE type was S2. Among these strains, higher rates of resistance to cefazolin (36/36), Ampicillin (9/36), streptomycin (2/36) were found. All strains were positive for tlh and negative for trh. The 31clinical isolates and 1 environmental isolate carried tdh gene. 4 known ST types, 2 new ST types and 23 core genome MLST(cgMLST) types were identified by sequence analysis. Thirty-four strains carried the resistant gene, mainly blaCARB-22. Conclusion Five foodborne disease outbreaks caused by Vibrio parahaemolyticus were occured in Shaoxing from 2022 to 2023. O10:K4 was the predominant serotypes of outbreaks. The isolates were all resistant to cefazolin. There is a high homogeneity between the strains of different outbreaks, indicating persistent sources of contamination ignored by traditional methods. It is necessary to strengthen the tracing and epidemiological investigation of Vibrio parahaemolyticus in this region.