Objective The determination of metal content of iron， copper and heavy metal element of lead in wine by inductively coupled plasma mass spectrometry （ICP-MS） was established.Methods The wine samples were digested by temperature programmed microwave digestion system. Helium gas was used as reaction gas to eliminate molecular ion interference， and the samples were determined by ICP-MS method. The influence of ⁵⁶Fe and ⁵⁷Fe isotopes on the determination results in collision mode and non-collision mode were verified. The differences of three pretreatment methods， microwave digestion， dilution and electrothermic digestion were compared.Results The parameters of the instrument were optimized and the detection limits of the three elements were 0.003-0.007 mg/L， precision of the method was 0.8%-5.1% and the recovery was 90.4%-106.0%. It was proved that the iron content in wine can be determined accurately by microwave digestion-⁵⁶Fe-collision mode or microwave digestion-⁵⁷Fe-non-collision mode. The direct dilution method can be used to determine elements of lead and copper quickly and accurately. Contents of iron and lead were determined with certified reference material and results were in the specified range.Conclusion The method has the advantages of high sensitivity， rapid and accurate detection results， and is suitable for the determination of iron， copper and lead in wine.