Objective To investigation and analysis the pollution status and dietary exposure risk of perfluoroalkyl and polyfluoroalkyl substances in animal-derived foods sold in Hunan Province. Methods From 2023 to 2024，a total of 266 samples of animal-derived foods including aquatic products、livestock meat、eggs、dairy products were randomly collected in Hunan Province. The content of 23 types of PFASs in the samples was determined by UPLC-MS/MS，and the dietary exposure of PFASs in animal derived foods was evaluated by the method of point estimation. Results 17 types of PFASs were detected in 202 samples，with a total detection rate of 75.9%. The overall detection rates of various types of food were ranked from high to low as follows: aquatic products (100%)>eggs (91.6%)> livestock meat (80.5%)>dairy products (24.2%)，and the difference was statistically significant (χ2=94.69, P<0.005)；the content range of 23 PFASs was <0.01~8.17ng/g，with the highest average PFOS content in aquatic products being 0.42ng/g，the HR evaluated by PFOA、PFOS、and PFBA monomers in various types of food was all less than 1. Conclusion The contamination of PFASs in animal derived food sold in Hunan Province was relatively common，but the degree of contaminationwais relatively light, and the impact on residents"" health was limited.