Objective This study aimed to prepare proficiency testing samples that are closer to the actual samples and have good homogeneity and stability， and the ability of national laboratories to detect procymidone， carbofuran， and parathion in vegetables was analyzed through proficiency testing.Methods The proficiency testing samples were prepared by adding the target standard solution to the vegetable juice that was sold in the market， and the uniformity and stability were tested. The assigned value and standard deviation of proficiency testing were obtained by robust statistics， and the z value was applied to evaluate the results submitted by the participating laboratories.Results The prepared samples were tested for homogeneity and stability， and met the proficiency testing requirements. The samples were applied to proficiency testing， and 94 laboratories participated nationwide， of which the laboratory satisfaction rate in the procymidone project was 84.5%； the laboratory satisfaction rate in the carbofuran project was 83.3%； the laboratory satisfaction rate in the parathion project was 89.6%.Conclusion The sample prepared by the preparation method in this study has favorable homogeneity and stability and can meet the requirements of the sample for proficiency testing. The statistical results of proficiency testing showed that the detection ability of procymidone， carbofuran， and parathion in vegetables used in participating laboratories was generally favorable. Through this capability verification activity， we gained an understanding of the testing level of laboratories in this field and achieved the expected results.