Objective To conduct laboratory analysis of a suspected foodborne disease outbreak caused by Cronobacter spp.， thereby providing a methodological reference for rapid and accurate source tracing of similar outbreaks.Methods Epidemiological data and clinical information from the outbreak were collected. Anal swab samples from affected individuals and kitchen staff， as well as samples of suspected contaminated food and environmental surfaces， were tested for diarrheal pathogens including Cronobacter spp. using quantitative PCR （qPCR）. Cronobacter positive samples were subjected for Cronobacter isolation. Cronobacter isolates were carried out for antimicrobial susceptibility testing， whole-genome sequencing （WGS）， and comprehensive bioinformatic analysis.Results In this event， a total of seven Cronobacter strains were isolated from two anal swabs of affected cases， one anal swab of a kitchen staff member， three environmental surface swabs， and one sample of suspected contaminated food. All isolates were identified as Cronobacter sakazakii sequence type ST1116 （a novel ST）. Pairwise core-genome single-nucleotide polymorphism （SNP） differences among the seven strains were ≤2， indicating high genetic relatedness.Conclusion This study provides the first evidence of clonal transmission of C. sakazakii ST1116 associated with a suspected foodborne disease outbreak.